The microscope is capable of observing three-dimensional microbiological structures as small as 0.4 μm×0.4 μm×1.0 μm (𝑥𝑦𝑧) using quasi-monochromatic light and a liquid crystal retarder.
Abstract
We developed an ultrahigh-resolution full-field optical coherence tomography (FF-OCT) microscope that is based on the spatial, rather than the temporal, coherence gating. The microscope is capable of observing three-dimensional microbiological structures as small as 0.4 μm×0.4 μm×1.0 μm (𝑥𝑦𝑧) using quasi-monochromatic light and a liquid crystal retarder. Unlike traditional FF-OCT systems, this microscope can be operated in high resolution for any preferable wavelength with minimized defocusing and dispersion effects. High-resolution images of an onion cell are presented.
©2012 Optical Society of America
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